Background: One of the deadliest forms of viral hepatitis, the hepatitis B virus, poses a severe threat to human health. Because they frequently come into contact with bodily secretions, especially blood, that are contaminated with the virus, health providers are frequently deals with that secretions. Precautions like personal safety measures and vaccinations can be avoided.

Objective: To determine the health providers knowledge, attitude, and practice about avoiding hepatitis B infection and getting the immunization against infection in Al- Salama- Amriya hospital, Baghdad.

Materials and Methods: A cross-sectional study including (154) health providers how work in different health institution in Amira Baghdad city included (Al Salama hospital (90 persons), primary care (44 persons) and specialist dental care centers (20 persons), During October and November 2022,. The information was collected by Using a questionnaire, included the information about the knowledge, attitude, and practice of health provider regarding the infection by Hepatitis B, immunization and demographic information. The study data were presented using a descriptive summary created in the excel software's 10 window utilizing frequencies, proportions, and percentages. Each subject gave their verbal consent, and the study kept the individuals' identities anonymous.

Results: Our study including (154) health providers are medical staff (59) and (95) paramedical staff, the overall knowledge scoring of them is (68%), overall attitude scoring is (75%), and the overall practice is (58%), among the medical staffs only (15%) had undergone screening for HBV, and (67%) had completed the three doses in other side the paramedical without history of screening for HBV with (37%) had completed the three doses.

Conclusion: Over all attitude of health providers is more than the knowledge and practice (75%), (68%) and (58%) respectively with low rate of vaccination specially paramedical staff.